Purification of Human Monoclonal Antibodies and Their Fragments, Methods in Molecular Biology, 2018 (Book chapter)
Methods for purification of human mAbs and fragments covering both batch and continuous chromatography approaches.
RESOURCE LIBRARY
The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.
The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.
Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)
First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.
Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)
Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.
Müller et al.
Biotechnol. Bioeng. (Open-Access)
Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.
Methods for purification of human mAbs and fragments covering both batch and continuous chromatography approaches.
A review of current state and future directions of continuous biochromatography discussing implementation challenges and opportunities for CaptureSMB and MCSGP technologies.
Comprehensive methodology for designing continuous expression and purification processes including perfusion bioreactors, CaptureSMB, and MCSGP.
Comparison of different Protein A resins in multi-column CaptureSMB configurations using mechanistic modeling provides guidance for resin selection in continuous capture.
CaptureSMB scale-up from laboratory to manufacturing scale establishes engineering principles for continuous capture process transfer.
Review of continuous integrated manufacturing concepts for therapeutic proteins discussing upstream-downstream integration and process control strategies.
A practical primer on chromatography: what it is, how it works, and why it matters. Learn about key separation mechanisms including reversed-phase, ion exchange, size exclusion and affinity chromatography, plus how to read chromatograms and the difference between analytical and preparative methods.
YMC ChromaCon Chromatography Newsletter.
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