Continuous Chromatography Resource Library

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Peer-Reviewed Publications 22 May 2020

From batch to continuous chromatographic purification of a therapeutic peptide through multicolumn countercurrent solvent gradient purification, J. Chromatogr. A

A simple procedure for developing MCSGP processes from single batch experiments is demonstrated for glucagon purification, achieving 90% yield at 90% purity with 23% yield improvement over batch.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 16 Apr 2020

Oligonucleotides: Current Trends and Innovative Applications in the Synthesis, Characterization, and Purification, Biotechnol. J.

A comprehensive review of oligonucleotide synthesis, characterization, and purification technologies highlighting MCSGP’s role in overcoming downstream processing bottlenecks.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 05 Feb 2020

Process-wide control and automation of an integrated continuous manufacturing platform for antibodies, Biotechnol. Bioeng.

Automated end-to-end integrated continuous manufacturing combines perfusion bioreactor, CaptureSMB, virus inactivation, and polish chromatography.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 31 Jan 2020

Design space and robustness analysis of batch and counter-current frontal chromatography processes for removal of antibody aggregates, J. Chromatogr. A

Systematic design space analysis comparing batch and MCSGP processes for aggregate removal establishes robustness metrics for continuous polishing operations.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 30 Jan 2020

Model-based process development of continuous chromatography for antibody capture: A case study with twin-column system, J. Chromatogr. A

Model-based approaches for twin-column continuous capture process design establish systematic methodology for CaptureSMB implementation.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 06 Jun 2019

Process for Continuous Fab Production by Digestion of IgG, Biotechnol. J.

A continuous process for Fab fragment production through enzymatic IgG digestion integrates upstream digestion with MCSGP purification.

Continuous Polishing (MCSGP)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

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