RESOURCE LIBRARY

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

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Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Peer-Reviewed Publications 26 Aug 2016

Optimal model-based design of the twin-column CaptureSMB process improves capacity utilization and productivity in protein A affinity capture, Biotechnol. J.

Optimal model-based design methodology for CaptureSMB demonstrates improved capacity utilization and productivity compared to empirical design approaches.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 06 Aug 2016

Process performance and product quality in an integrated continuous antibody production process, Biotechnol. Bioeng.

Analysis of process performance and product quality across integrated continuous mAb production demonstrates stable operation under varying conditions.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 04 Jul 2016

Continuous counter-current chromatography for capture and polishing steps in biopharmaceutical production, Biotechnol. J.

A comprehensive review of twin-column continuous chromatography technologies including CaptureSMB and MCSGP for biopharmaceutical capture and polishing applications.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 22 Mar 2016

Model based adaptive control of a continuous capture process for monoclonal antibodies production, J. Chromatogr. A

Model-based adaptive control for CaptureSMB automatically adjusts operating conditions in response to feed titer variations.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 15 Mar 2016

Comparison of batch and continuous multi-column protein A capture processes by optimal design, Biotechnol. J.

Numerical optimization comparing CaptureSMB, 3-column, and 4-column PCC with batch capture finds CaptureSMB optimal for most production scenarios.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 01 Apr 2015

Twin-Column Cation-Exchange Chromatography for the Purification of Biomolecules, Biopharm International

Twin-column cation-exchange MCSGP for biomolecule purification showcases yield and productivity improvements over batch processing.

Continuous Polishing (MCSGP)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

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