Contichrom® TWIN 300 LPLC - Capture

The Contichrom® TWIN 300 LPLC – Capture is a sanitary chromatography system for continuous capture of biologics from pilot to large-scale GMP manufacturing. Featuring patented CaptureSMB® technology with AutomAb® dynamic control, the system maximizes resin binding capacity to deliver up to 3× faster processing and 50% less Protein A resin and buffer consumption compared to batch chromatography. Purpose-built for monoclonal antibodies, viral vectors (AAV), and recombinant proteins, the system features a fully sanitary, CIP-ready flow path in SS 316L or Hastelloy with a 7.5-bar maximum operating pressure. The TWIN 300 has flow rates from 0.03 – 3.33 L/min.

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Overview

GMP-Ready Continuous Capture for Biopharmaceutical Manufacturing

The Contichrom® TWIN LPLC – Capture is a sanitary chromatography system for continuous capture of biologics from pilot to large-scale GMP manufacturing.

Twin-column continuous capture (CaptureSMB® with AutomAb®)
Up to 3× faster processing, 50% less resin and buffer vs. batch
Sanitary design with CIP capability for biopharmaceutical production
SS 316L or Hastelloy flow paths, 7.5 bar maximum operating pressure
21 CFR Part 11 compliant software with full audit trail

One System, Multiple Processes

Batch AND continuous capture on a single sanitary skid:

Mode	Technology
Batch	Single-column isocratic and gradient
2D Integrated Batch	Two-step purification with in-line dilution (BID)
Continuous Capture	CaptureSMB® with AutomAb®

Switch seamlessly between batch and CaptureSMB® modes
Buffer in-line dilution (BID) option for on-board pH adjustment
Eliminates duplicate equipment — saves space and capital

Patented CaptureSMB Technology

Twin-column continuous capture maximizing resin binding capacity:

Metric	Value
Speed	Up to 3× faster than batch
Resin & buffer	Up to 50% reduction
Productivity	2–4× vs. single-column batch

Two columns alternate loading and elution — continuous output
Columns in series so the second captures breakthrough from the first
Near-complete resin utilization eliminates re-chromatography

AutomAb® - Dynamic Process Control

AutomAb® monitors UV breakthrough in real time and adjusts column switching automatically:

Detects product breakthrough and triggers switching at the optimal point
Compensates for feed variability, column aging, and resin degradation
Delivers consistent capture efficiency without operator intervention
Enables robust, continuous 24/7 manufacturing
Steady state reached after only one cycle with <1% variability

Comprehensive Application Range

Production-scale capture for biopharmaceuticals:

Application	CaptureSMB®	Integrated Batch
mAbs & Antibody Variants	✓	✓
Viral Vectors (AAV)	✓	✓
Antibody-drug Conjugates	✓	✓
Recombinant Proteins	✓	✓

All processes run on the same sanitary hardware
AutomAb® dynamic control across continuous operations

Scalable from Lab to Production

Part of the Contichrom® platform with established transfer protocols:

System	Flow Rate	Column ID
CUBE 30/100	0.1–100 mL/min	0.5–5 cm
TWIN LPLC 300	0.03 – 3.33 L/min	10–20 cm
TWIN LPLC 500	0.08 – 8.33 L/min	20–45 cm
TWIN LPLC 1000	0.15 – 18.9 L/min	30–60 cm
TWIN LPLC 2000	0.5 – 36 L/min	60–100 cm

Scale-up from CUBE at over 100× verified and published
Same CaptureSMB® and AutomAb® control at every scale

Sanitary Design for Bioprocessing

Purpose-built for biopharmaceutical manufacturing environments:

Fully sanitary flow path with drainable design and CIP compatibility
ASME-BPE compliant stainless steel welds on all product contact surfaces
SS 316L or Hastelloy flow path materials
SF5 spec product contact tubing (≤ 20 Ra EP)
Designed for NaOH 1M CIP and compatible with single-use aseptic interfaces
Maximum operating pressure of 7.5 bar — optimal for affinity and LP resins

Designed for GMP Compliance

Built to meet stringent biopharmaceutical manufacturing requirements:

Developed under the GAMP 5 framework
21 CFR Part 11 compliant software with audit trail
Comprehensive Turnover Package (TOP) with ASME-BPE compliant welds
USP Class VI materials and FDA-approved seals
UL-approved panels, CE marking including PED compliance

TOP includes: MTRs, pressure test reports, passivation reports, weld documentation, as-built drawings.

Production-Grade Hardware

A complete skid-mounted system with mobile frame:

2× Pumps (base) — LEWA ecodos triplex with rupture detection
3rd Pump (BID option) — Buffer in-line dilution
Dual UV Detectors — 4-Channel Adjustable wavelength (200-600 nm), 0.5 mm path length
2× Coriolis flow meters + 8× pressure transmitters
pH, conductivity & temperature — Post-column standard
Air sensors — Pre-pump and pre-column
Pneumatic valves — Sanitary design, fast-acting at low pressure
24″ HMI — Industrial touchscreen with FactoryTalk View SE

CIP-Ready Design

The Contichrom® TWIN LPLC – Capture is designed for cleaning-in-place:

Dedicated CIP inlet and outlet manifolds integrated into the valve network
Fully drainable flow path design minimizes carryover between campaigns
Compatible with NaOH 1M CIP protocols
Optional single-use aseptic interface for minimally classified suites
Reduces turnaround time and supports multi-product facility operation

How it works

Sanitary Hardware for GMP Biopharmaceutical Capture Chromatography

The Contichrom TWIN LPLC – Capture is a complete sanitary skid-mounted unit for GMP manufacturing:

Mobile frame with levelers and non-marking caster wheels
316L stainless steel frame with mechanically polished welds
Sanitary flow path designed for drainability and CIP
Two column positions for CaptureSMB and single-column batch
Operating temperature: 4–25 °C ambient, 4–40 °C process

LEWA Ecodos Triplex Pumps

Industry-leading LEWA ecodos triplex pumps for precision and reliability:

Pump	Role
Pump A	Feed delivery / Load buffer
Pump B	Elution / Wash / Equilibration
Pump C (optional)	Buffer in-line dilution (BID)

Triplex configuration for nearly pulse-free flow
Sandwich diaphragm with rupture detection alarm
Automatic stroke adjustment for optimal performance
PTFE diaphragms — USP Class VI certified, FDA approved
Base system includes 2 pumps; 3rd pump available with BID option

Pneumatic Valve Network

Sanitary pneumatic valves — fast-acting at low pressure:

Inlet Selection Valves — Multiple ports per pump for automated buffer/feed switching
Column Switching Valves — For CaptureSMB interconnected and parallel configurations
Fraction Outlet Valves — Product and waste routing with drain connection
CIP Manifolds — Dedicated inlet and outlet for cleaning-in-place
Materials: 316L SS body, PTFE/EPDM diaphragm, SF5 spec product contact (≤ 20 Ra EP)
Sanitary design

Dual Wavelength UV Detectors

Two post-column UV sensors for real-time monitoring and AutomAb® control:

Parameter	Specification
Type	4-channel, multi-wavelength DAD, 200–600 nm
Path length	0.5 mm path (exchangeable for 0.1, 0.3, or 1 mm)

Provides the UV breakthrough signals that enables AutomAb® dynamic process control
Critical for consistent CaptureSMB® capture efficiency

In-Line Process Sensors

Comprehensive real-time monitoring:

Sensor	Quantity	Specification
Coriolis Flow Meters	2	Flow monitoring and control
Pressure Transmitters	8	0–13.8 bar, ±1% FS
pH	Post-column (x2)	0–13 pH, ±0.15 unit
Conductivity	Post-column (x2)	0–200 mS/cm, ±1% of measured value
Temperature	Per column	0–100 °C, ±0.25 °C at 25 °C
Air Sensors	Pre-pump + pre-column	Ultrasonic detection

Column Management

Flexible column configurations for production scale:

2 column positions for CaptureSMB® twin-column operation
Column ID range: 10–100 cm (depending on model)
Low-pressure operation Max 7.5 bar — optimized for affinity resins
Sanitary tri-clamp connections for column attachment
Single-column batch mode available

Human-Machine Interface (HMI)

System control via industrial PC running FactoryTalk View SE:

Component	Specification
Touchscreen	24″ industrial HMI, panel-mounted
Industrial PC	Preloaded with control software
OS	Windows 11 Professional

Real-time process visualization, recipe management, batch reporting

GMP-Grade Flow Path

Every product-contact surface selected for biopharmaceutical compatibility:

Component	Materials
Product Contact Tubing	316L SS or Hastelloy, ≤ 20 Ra EP (SF5 spec)
Non-Product Contact Tubing	316L SS, ≤ 20 Ra MP (SF1 spec)
Process Valves	316L SS body, PTFE/EPDM diaphragm
Pump Diaphragms	PTFE (USP Class VI, FDA approved)

ASME-BPE compliant welds, drainable design, CIP-compatible
Surface roughness < 0.8 µm Ra on wetted metallic parts

PLC-Based Control System

Industrial automation platform:

Component	Specification
PLC Platform	Rockwell CompactLogix
HMI Runtime	FactoryTalk View SE
Process Control	CaptureSMB with AutomAb®
Database	Microsoft SQL Server
Historian	YMC Server Historian
Reports	YMC Server Reports (auto-generated)
Remote Access	HMS Ewon module (client-enabled)

Optional Hardware

Option	Specification
Buffer In-line Dilution (BID)	Pump C + inlet valves + static mixer + sensors
Additional Inlet Valves	Up to 6 per pump
Additional Outlet Valves	Up to 5 fraction + 1 waste
Filter Housings	Single or dual with valve manifolds
Bubble Traps	2 with valve manifold and vent valves
CIP Manifolds	Aseptic single-use bag connection
Software Customizations	Handshake with upstream/downstream equipment, additional control loops, external valve/equipment control

Process Capabilities

Continuous Capture for Biopharmaceutical Production

The Contichrom TWIN LPLC – Capture is optimized for twin-column continuous capture:

CaptureSMB® with AutomAb® — Continuous capture
2D Integrated Batch — Two-step purification with in-line dilution (BID option)
Batch — Single-column isocratic and gradient operation
All accessible from a single sanitary GMP-ready platform

CaptureSMB® with AutomAb® — Continuous Capture

Twin-column continuous capture maximizing resin binding capacity:

Two identical columns alternate between loading and elution
Columns in series — second column captures breakthrough from the first
AutomAb® detects UV breakthrough and triggers column switching
Near-complete utilization of resin binding capacity
Continuous feed processing eliminates batch size limitations
Steady state after one cycle with <1% variability

Target: Affinity capture steps (Protein A, mixed-mode) Molecules: mAbs, antibody variants, viral vectors (AAV), ADCs, recombinant proteins

CaptureSMB® — Performance Gains

Metric	Result
Processing speed	Up to 3× faster than batch
Protein A resin consumption	Up to 50% reduction
Buffer consumption	Up to 50% reduction
Productivity	2–4× higher vs. single-column batch
Intermediate storage	Reduced — continuous output eliminates hold steps
Process control	AutomAb® enables continuous 24/7 operation
Scale-up variability	<1% — steady state after one cycle

One column loads while the other elutes — continuous output, maximum resin utilization, consistent quality.

Integrated Batch — Two-Step Purification

Couple two chromatography steps without intermediate handling:

Parallel or in-series column configurations
Two chromatography modes run consecutively (e.g., IEX then HIC)
On-board buffer in-line dilution (BID option) for pH adjustment
Eliminates intermediate hold tanks and manual handling
Reduces process time, QC burden, and facility footprint

Molecules: mAbs, antibody variants, viral vectors (AAV), ADCs, recombinant proteins

Batch Chromatography

Single-column batch purification:

Mode	Applications
Isocratic	Affinity capture, SEC, desalting
Gradient	IEX, HIC, mixed-mode polishing

Linear gradient from 5% to 95% in batch mode
Multiple fraction collection outlets
Full UV/flow/pressure/pH/conductivity monitoring

Buffer In-line Dilution (BID)

On-board dilution of buffer concentrates and CIP solutions:

Third pump (Pump C) delivers dilution buffer at controlled ratio
Static mixer ensures homogeneous blending
Enables pH adjustment between capture and polish steps
Supports sequential processing for pool-less multi-step chromatography

Requires: BID option (Pump C, inlet valves, static mixer, sensors)

Regulatory Compliance & Documentation

Certifications

UL-approved electrical panels
CE marking available including PED compliance
Developed under GAMP 5 framework
Complies with FDA 21 CFR Part 11 regulations

Standards & Directives

ASME-BPE for all stainless steel product contact welds
FDA-approved USP Class VI materials and seals
UL-approved electrical panels
CE marking with PED compliance

Materials & Documentation

All wetted materials selected for biopharmaceutical compatibility and chemical resistance
Full traceability with MTRs and certificates of compliance
Product contact surface roughness: < 0.8 µm Ra (SF5 spec: ≤ 20 Ra EP)
Turnover Package (TOP) with comprehensive QA/QC documentation

TOP includes: MTRs, certificates of compliance, pressure test reports, passivation reports, weld documentation (logs, inspection reports, procedures, coupon logs, isometrics), slope verification, operation and maintenance manuals, as-built drawings (PDF and native CAD/Solidworks).

Specifications

System Models

Parameter	TWIN 300	TWIN 500	TWIN 1000	TWIN 2000
Flow Rate	0.03 – 3.33 L/min	0.08 – 8.33 L/min	0.15 – 18.9 L/min	0.5 – 36 L/min
Column ID	10–20 cm	20–45 cm	30–60 cm	60–100 cm
Max Operating Pressure	7.5 bar	7.5 bar	7.5 bar	7.5 bar
Best For	Pilot / clinical	Mid-scale GMP	Large-scale GMP	Maximum capacity

General Specifications

Specification	Value
Max Operating Pressure	7.5 bar (0.75 MPa / 109 psi)
Operating Temperature	4–25 °C ambient; 4–40 °C process
Design	Sanitary, CIP-compatible (NaOH 1M)
Wetted Materials	SS 316L or Hastelloy, PTFE, EPDM (USP Class VI)
Surface Finish	< 0.8 µm Ra (product contact), SF5 spec: ≤ 20 Ra EP
Control System	Rockwell CompactLogix PLC
Processes	Batch, Integrated Batch, CaptureSMB®

Physical Specifications

Model	Length	Width	Height	Approx. Weight
TWIN 300	218 cm	127 cm	203 cm	2494 kg
TWIN 500	299 cm	165 cm	198 cm	3055 kg
TWIN 1000 (Pump)	360 cm	140 cm	230 cm	4455 kg
TWIN 1000 (Process)	140 cm	90 cm	200 cm	1364 kg
TWIN 2000 (Pump)	381 cm	152 cm	230 cm	4763 kg
TWIN 2000 (Process)	147 cm	101 cm	200 cm	1543 kg

Pumps (LEWA ecodos)


Configuration	2× triplex diaphragm pumps (3× with BID option)
Diaphragm	Sandwich PTFE with rupture detection
Materials	USP Class VI certified, FDA approved
Features	Automatic stroke adjustment, nearly pulse-free flow

Detectors & Sensors


UV Detection	4-channel, multi-wavelength DAD, 200–600 nm, 0.5 mm path (exchangeable for 0.1, 0.3, or 1 mm)
Flow Meters (×2)	Coriolis
Pressure (×8)	Transmitters throughout flow path, 0–13.8 bar, ±1% FS
pH	On waste and collection outlets, 0–13, ±0.15 unit
Conductivity	On waste and collection outlets, 0–200 mS/cm, ±1% of measured value
Temperature	Post-column, 0–100 °C, ±0.25 °C at 25 °C
Air Sensors	Pre-pump and pre-column (ultrasonic)

Valves & Flow Path


Valve Actuation	Pneumatic diaphragm valves
Valve Materials	316L SS body, PTFE/EPDM diaphragm
Product Contact	SF5 spec: ≤ 20 Ra EP
Non-Product Contact	SF1 spec: ≤ 20 Ra MP
Column Connections	Sanitary tri-clamp
CIP	Dedicated inlet and outlet manifolds

Software & Automation


PLC Platform	Rockwell CompactLogix
HMI Runtime	FactoryTalk View SE
Process Control	CaptureSMB® with AutomAb®
Database	Microsoft SQL Server
Historian	YMC Server Historian
Reports	YMC Server Reports (auto-generated)
Remote Access	HMS Ewon module (client-enabled)
Data Integrity	21 CFR Part 11, audit trail, electronic signatures

User Interface


HMI	24″ industrial touchscreen, panel-mounted
Industrial PC	Preloaded with control software
OS	Windows 11 Professional

Optional Modules

Option	Specification
Buffer In-line Dilution (BID)	Pump C + inlet valves + static mixer + sensors
Additional Inlet Valves	Up to 6 per pump
Additional Outlet Valves	Up to 5 fraction + 1 waste
Filter Housings	Single or dual with valve manifolds
Bubble Traps	2 with valve manifold and vent valves
CIP Manifolds	Aseptic single-use bag connection
Software Customizations	Handshake with upstream/downstream equipment, additional control loops, external valve/equipment control

Technologies

Applications

Resources

Service & Support

FAQ

What is included in a standard system?

A complete Contichrom® TWIN LPLC – Capture system includes:

Complete sanitary skid assembly with mobile frame (levelers and casters)
2× LEWA ecodos triplex pumps with sandwich diaphragms and rupture detection
Full inlet and outlet pneumatic valve network (sanitary design)
Dual wavelength UV detectors (280 nm, 303 nm)
Post-column pH and conductivity sensors
2× Coriolis flow meters + 8× pressure transmitters
Pre-pump and pre-column air sensors
CIP inlet and outlet manifolds
24″ HMI with industrial PC and control software preloaded
Rockwell CompactLogix PLC control system
Comprehensive TOP documentation package
Factory Acceptance Testing (FAT) — up to 5 days
One week on-site startup support and training

Customer provides: Columns, filter elements, chromatography resin, external piping, utilities connections.

How does this differ from the TWIN HPLC and TWIN LPLC Polishing?

Feature	TWIN HPLC	TWIN LPLC Capture	TWIN LPLC Polishing
Design	Industrial (non-sanitary)	Sanitary	Sanitary
Pressure	80 bar	7.5 bar (20 bar opt.)	7.5 bar (20 bar opt.)
Core Process	MCSGP (polishing)	CaptureSMB® (capture)	MCSGP (polishing)
HP Valves	YMC fast-acting	Standard pneumatic	Standard pneumatic
Pumps	4× LEWA ecoflow	2× (3×) LEWA ecodos	4× LEWA ecodos
Molecules	Synthetic peptides, oligos	Biologics (mAbs, AAV, ADCs)	Biologics (mAbs, AAV, ADCs)

The TWIN LPLC – Capture is the right choice for affinity capture of biopharmaceuticals where sanitary design and CIP capability are required.

Which system size do I need?

Model	Column ID	Flow Rate	Best For
TWIN 300	10–20 cm	0.03 – 3.33 L/min	Pilot production, clinical manufacturing
TWIN 500	20–45 cm	0.08 – 8.33 L/min	Mid-scale commercial production
TWIN 1000	30–60 cm	0.15 – 18.9 L/min	Large-scale commercial production
TWIN 2000	60–100 cm	0.5 – 36 L/min	Maximum capacity production

Contact YMC to discuss your throughput requirements and optimal system selection.

What are the installation requirements?

Floor space: Dimensions vary by model (contact YMC for GA drawings)
Power: Model-dependent (contact YMC for specifications)
Utilities: Process air, water, compressed air, drain, network connection
Ambient temperature: 4–25 °C
Process temperature: 4–40 °C

Note: System must be uncrated, leveled, and connected to utilities before YMC on-site work begins.

How do I transfer methods from the CUBE?

Methods developed on Contichrom CUBE transfer directly to the TWIN LPLC
Same CaptureSMB® principles and AutomAb® control at both scales
Scale-up at over 100× verified and published
Steady state after one cycle with <1% variability
YMC ChromaCon provides process transfer support

What training and support is provided?

FAT — Up to 5 days at YMC facility
On-site startup — One week including commissioning and system walkdown
Training — 1 full day or 2 half-days covering operation and maintenance
Remote support — HMS Ewon remote access for troubleshooting and training

What is the warranty and service coverage?

Warranty: 12 months from installation or 18 months from shipment (whichever first)
Global service: YMC operations worldwide, LEWA support in 80 countries
Remote access: Built-in capability for automation troubleshooting
Components: Non-proprietary with global distribution for local acquisition
Maintenance: No special tools required for standard maintenance

Can the system be customized?

Yes — customization options include:

Buffer in-line dilution (BID) with additional pump
Additional inlet valves (up to 6 on Pump A, B, or C)
Additional outlet/fraction valves (up to 5 fraction + 1 waste)
Pre-column analytics (pH and conductivity)
Single or dual filter housings
Bubble traps
CIP manifolds for aseptic single-use bag connection

Contact YMC ChromaCon for custom configuration pricing.

Overview

GMP-Ready Continuous Capture for Biopharmaceutical Manufacturing

The Contichrom® TWIN LPLC – Capture is a sanitary chromatography system for continuous capture of biologics from pilot to large-scale GMP manufacturing.

Twin-column continuous capture (CaptureSMB® with AutomAb®)
Up to 3× faster processing, 50% less resin and buffer vs. batch
Sanitary design with CIP capability for biopharmaceutical production
SS 316L or Hastelloy flow paths, 7.5 bar maximum operating pressure
21 CFR Part 11 compliant software with full audit trail