RESOURCE LIBRARY

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

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Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Peer-Reviewed Publications 25 Aug 2026

Counter-Current Chromatography Enables Use of Green Solvents for Productive Peptide Purification Processes

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

Continuous Polishing (MCSGP)

White Papers 25 Aug 2026

Manufacturing advantages and cost savings of MCSGP/AutoPeak® for large-scale peptide purification

A techno-economic comparison of continuous twin-column purification against batch reversed-phase chromatography, across annual outputs from 10 kg to 10,000 kg of peptide API.

Continuous Polishing (MCSGP)

Application Notes 16 Feb 2026

Purification of a Therapeutic Oligonucleotide Using Twin-Column Chromatography (MCSGP)

This application note demonstrates a significant improvement in yield and productivity by converting an optimized batch chromatography method to Multi-column Counter-current Solvent Gradient Purification (MCSGP) for the manufacturing of a therapeutic oligonucleotide.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 06 Dec 2025

Process Characterization and Performance Qualification of MCSGP, Processes Journal

First publication presenting a Process Characterization and Process Performance Qualification approach for regulatory-compliant Process Validation of MCSGP, demonstrated for Bivalirudin purification.

Continuous Polishing (MCSGP)

Application Notes 04 Dec 2025

AutomAb®: UV-based Dynamic Process Control for CaptureSMB

“AutomAb is a UV-based dynamic process control method to adjust the CaptureSMB process following decline in binding capacity of the chromatography medium or changes of product compound concentration in the feed.”

Continuous Capture (CaptureSMB®)

Application Notes 04 Dec 2025

CaptureSMB for the continuous purification of mAbs with high productivity and load

CaptureSMB is a twin column periodic countercurrent (PCC) process used for the continuous purification of monoclonal antibodies (mAbs) and antibody fragments using affinity resins such as Protein A and Protein L.

Continuous Capture (CaptureSMB®)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

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