Continuous Chromatography Resource Library

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Peer-Reviewed Publications 16 Apr 2020

Oligonucleotides: Current Trends and Innovative Applications in the Synthesis, Characterization, and Purification, Biotechnol. J.

A comprehensive review of oligonucleotide synthesis, characterization, and purification technologies highlighting MCSGP’s role in overcoming downstream processing bottlenecks.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 31 Jan 2020

Design space and robustness analysis of batch and counter-current frontal chromatography processes for removal of antibody aggregates, J. Chromatogr. A

Systematic design space analysis comparing batch and MCSGP processes for aggregate removal establishes robustness metrics for continuous polishing operations.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 06 Jun 2019

Process for Continuous Fab Production by Digestion of IgG, Biotechnol. J.

A continuous process for Fab fragment production through enzymatic IgG digestion integrates upstream digestion with MCSGP purification.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 30 Mar 2019

Experimental Evaluation of the Impact of Intrinsic Process Parameters on the Performance of a Continuous Chromatographic Polishing Unit (MCSGP), Biotechnol. J.

Systematic evaluation of MCSGP process parameters for antibody polishing identifies critical variables affecting separation performance and product quality.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 12 Dec 2018

Purification of Human Monoclonal Antibodies and Their Fragments, Methods in Molecular Biology, 2018 (Book chapter)

Methods for purification of human mAbs and fragments covering both batch and continuous chromatography approaches.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 16 Oct 2018

Current status and future challenges in continuous biochromatography, Curr. Opin. Chem. Eng.

A review of current state and future directions of continuous biochromatography discussing implementation challenges and opportunities for CaptureSMB and MCSGP technologies.

Continuous Capture (CaptureSMB®)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

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