Twin-Column Cation-Exchange Chromatography for the Purification of Biomolecules, Biopharm International
Twin-column cation-exchange MCSGP for biomolecule purification showcases yield and productivity improvements over batch processing.
RESOURCE LIBRARY
The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.
The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.
Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)
First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.
Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)
Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.
Müller et al.
Biotechnol. Bioeng. (Open-Access)
Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.
Twin-column cation-exchange MCSGP for biomolecule purification showcases yield and productivity improvements over batch processing.
Introduction of CaptureSMB twin-column continuous capture demonstrates superior productivity, capacity utilization, and 10-30% resin cost reduction versus batch chromatography.
Twin-column continuous chromatography applied for F(ab’)2 fragment capture demonstrates the technology’s applicability to non-mAb biotherapeutics.
Twin-column continuous capture technology increases Protein A resin capacity utilization from 50-60% to near-maximum levels.
Twin-column continuous chromatography presented as a productivity-enhancing technology for biopharmaceutical purification processes.
MCSGP applied for purification of common light-chain bispecific antibodies addresses the unique separation challenges posed by this antibody format.
A practical primer on chromatography: what it is, how it works, and why it matters. Learn about key separation mechanisms including reversed-phase, ion exchange, size exclusion and affinity chromatography, plus how to read chromatograms and the difference between analytical and preparative methods.
YMC ChromaCon Chromatography Newsletter.
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