Experimental design of a twin-column countercurrent gradient purification process, J. Chromatogr. A
Experimental design methodology for twin-column MCSGP processes establishes systematic approaches for process development and optimization.
RESOURCE LIBRARY
The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.
The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.
Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)
First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.
Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)
Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.
Müller et al.
Biotechnol. Bioeng. (Open-Access)
Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.
Experimental design methodology for twin-column MCSGP processes establishes systematic approaches for process development and optimization.
Optimal model-based design methodology for CaptureSMB demonstrates improved capacity utilization and productivity compared to empirical design approaches.
Analysis of process performance and product quality across integrated continuous mAb production demonstrates stable operation under varying conditions.
A comprehensive review of twin-column continuous chromatography technologies including CaptureSMB and MCSGP for biopharmaceutical capture and polishing applications.
Model-based adaptive control for CaptureSMB automatically adjusts operating conditions in response to feed titer variations.
Numerical optimization comparing CaptureSMB, 3-column, and 4-column PCC with batch capture finds CaptureSMB optimal for most production scenarios.
A practical primer on chromatography: what it is, how it works, and why it matters. Learn about key separation mechanisms including reversed-phase, ion exchange, size exclusion and affinity chromatography, plus how to read chromatograms and the difference between analytical and preparative methods.
YMC ChromaCon Chromatography Newsletter.
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