Online control of the twin-column countercurrent solvent gradient process for biochromatography, J. Chromatogr. A
Online control strategies for twin-column MCSGP processes enable real-time process monitoring and automated optimization.
RESOURCE LIBRARY
The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.
The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.
Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)
First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.
Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)
Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.
Müller et al.
Biotechnol. Bioeng. (Open-Access)
Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.
Online control strategies for twin-column MCSGP processes enable real-time process monitoring and automated optimization.
Extension of MCSGP to multifraction separations demonstrates purification of multiple intermediate-eluting species in a single continuous operation.
Development and validation of mechanistic models for cation-exchange antibody capture comparing batch and MCSGP process performance predictions.
A two-step MCSGP process for IgG2 capture and purification demonstrates integrated continuous downstream processing.
MCSGP demonstrated for enrichment of active antibody variants shows how continuous chromatography can improve therapeutic protein quality attributes.
Model predictive control strategies for MCSGP optimization enable advanced process control and real-time optimization.
A practical primer on chromatography: what it is, how it works, and why it matters. Learn about key separation mechanisms including reversed-phase, ion exchange, size exclusion and affinity chromatography, plus how to read chromatograms and the difference between analytical and preparative methods.
YMC ChromaCon Chromatography Newsletter.
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