Brochures & Datasheets
04 Dec 2025
RESOURCE LIBRARY
Continuous Chromatography Resources — Application Notes, Webinars & Papers
The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.
Recent MCSGP Research Highlights
The first peer-reviewed PC/PPQ framework for MCSGP
The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.
Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)
First peer-reviewed AIEX MCSGP for therapeutic siRNA
First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.
Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)
Proof-of-concept MCSGP for AAV2 polishing
Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.
Müller et al.
Biotechnol. Bioeng. (Open-Access)
Resource Library
Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.
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Application Notes
03 Dec 2025
Highly pure Cannabidiol (CBD) by twin-column chromatography (MCSGP)
This application note shows how highly pure CBD with a THC content below 100 ppm was obtained from pre-treated hemp plant extract. The process provided a yield of 95%, an increase of 72% relative to single column batch chromatography.
Application Notes
03 Dec 2025
Purification of ADCs using Continuous Chromatography (MCSGP)
MCSGP was used for the purification of Antibody Drug Conjugates (ADCs) with various Drug-Antibody-Ratios (DARs) for a non-specifically conjugated model system, representing the Antibody-Drug-Conjugate Trastuzumab Emtansine (Kadcyla®).
Brochures & Datasheets
03 Dec 2025
Contichrom PILOT 300X Brochure
Overview of the technical specifications and functionalities offered by the Contichrom PILOT 300X
Peer-Reviewed Publications
16 Jul 2025
UV-based dynamic control improves the robustness of multicolumn countercurrent solvent gradient purification of oligonucleotides, Biotechnol. J.
AutoPeak, a UV-based dynamic process controller, automatically adjusts recycling and collection windows in MCSGP to maintain product quality during extended continuous operations.
Peer-Reviewed Publications
07 Jul 2025
Separation of Empty and Full Adeno-Associated Viral Capsids by MCSGP: From Model-Based Design to Implementation, J. Chromatogr. A
Model-based optimization of anion exchange separation of empty and full AAVs demonstrates that MCSGP increases full recovery 5-fold and productivity 2.5-fold while reducing process mass intensity to one-third compared to batch.
Educational Articles
Oligo Purification
07 Sep 2026
Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides
Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.
Oligo Purification
27 Aug 2026
Continuous Anion Exchange Purification of a Conjugated siRNA
An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.
Peptide Purification
26 Aug 2026
MCSGP Process Validation: Characterization and PPQ at Commercial Scale
Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.
Peptide Purification
25 Aug 2026
Acetonitrile- and TFA-Free Peptide Purification with MCSGP
Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.
YMC ChromaCon Chromatography Newsletter.
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