Continuous Chromatography Resource Library

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Peer-Reviewed Publications 12 Dec 2021

Purification of a GalNAc-cluster-conjugated oligonucleotide by reversed-phase twin-column continuous chromatography, J. Chromatogr. A

MCSGP applied for reversed phase purification of a GalNAc-conjugated DNA-LNA gapmer therapeutic increases yields from 52.7% to 91.5% while enabling 42.5% synthesis scale-down.

Continuous Polishing (MCSGP)

Webinars & Videos 06 Jul 2021

Continuous Chromatography of Peptides

Bachem webinar about the “Continuous Chromatography of Peptides” using MCSGP

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 10 Jun 2021

Experimental Design of the MCSGP Unit for the Separation of PEGylated Proteins, Ind. Eng. Chem. Res.

A model-based approach for MCSGP design applied to PEGylated protein separation improves yield and productivity by 8.2% and 17.8% respectively versus batch processing.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 03 May 2021

Process Intensification for the Purification of Peptidomimetics: The Case of Icatibant through MCSGP, Ind. Eng. Chem. Res.

MCSGP applied for Icatibant peptidomimetic purification demonstrates significant yield improvements and establishes economic viability across manufacturing scenarios.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 05 Oct 2020

Modern trends in downstream processing of biotherapeutics through continuous chromatography: The potential of Multicolumn Countercurrent Solvent Gradient Purification, Trends Anal. Chem.

A comprehensive review of continuous chromatography technologies for biotherapeutic downstream processing, with detailed analysis of MCSGP principles, applications, and future potential.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 22 May 2020

From batch to continuous chromatographic purification of a therapeutic peptide through multicolumn countercurrent solvent gradient purification, J. Chromatogr. A

A simple procedure for developing MCSGP processes from single batch experiments is demonstrated for glucagon purification, achieving 90% yield at 90% purity with 23% yield improvement over batch.

Continuous Polishing (MCSGP)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

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