Continuous Chromatography Resource Library

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

Download Paper

First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

Download Paper

Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

Download Paper

Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Application Notes 16 Feb 2026

MCSGP Process Development – Part 2: MCSGP Optimization

This application note presents a systematic approach for optimization of MCSGP with regards to the performance parameters Yield, Productivity and Eluent Consumption, and gives recommendations on the priority of the parameters.

Continuous Polishing (MCSGP)

Application Notes 16 Feb 2026

MCSGP Process Development – Part 1: Gradient Development

This application note provides a simple step-bystep flowchart template to guide the development of batch gradient conditions that result in optimal input data for the MCSGP wizard.

Dynamic Process Control (AutoPeak® & AutomAb®)

Brochures & Datasheets 04 Dec 2025

Contichrom CUBE Brochure

Batch Chromatography

Peer-Reviewed Publications 07 Jul 2025

Separation of Empty and Full Adeno-Associated Viral Capsids by MCSGP: From Model-Based Design to Implementation, J. Chromatogr. A

Model-based optimization of anion exchange separation of empty and full AAVs demonstrates that MCSGP increases full recovery 5-fold and productivity 2.5-fold while reducing process mass intensity to one-third compared to batch.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 04 Jul 2025

Enrichment of Full AAV2 Using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP), Biotechnol. Bioeng.

A proof-of-concept twin-column MCSGP process enhances the ratio of full to empty AAV capsids, increasing full capsid content from 30% to 68% while improving productivity by 23%.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 01 May 2023

Critical challenges and advances in recombinant adeno-associated virus (rAAV) biomanufacturing, Biotechnol. Bioeng.

A review of current challenges in rAAV manufacturing discussing how continuous chromatography technologies like MCSGP can address full/empty capsid separation.

Continuous Polishing (MCSGP)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

See all articles

YMC ChromaCon Chromatography Newsletter.
Subscribe for New Resources Unsubscribe anytime.