Brochures & Datasheets
04 Dec 2025
RESOURCE LIBRARY
Continuous Chromatography Resources — Application Notes, Webinars & Papers
The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.
Recent MCSGP Research Highlights
The first peer-reviewed PC/PPQ framework for MCSGP
The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.
Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)
First peer-reviewed AIEX MCSGP for therapeutic siRNA
First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.
Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)
Proof-of-concept MCSGP for AAV2 polishing
Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.
Müller et al.
Biotechnol. Bioeng. (Open-Access)
Resource Library
Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.
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Peer-Reviewed Publications
15 Dec 2022
Obtaining acidic and basic charge variants using a twin-column continuous chromatography system, Protein Expr. Purif.
A twin-column continuous chromatography method enables simultaneous separation and collection of both acidic and basic mAb charge variants in a single continuous operation.
Peer-Reviewed Publications
15 Oct 2022
Enrichment and recovery of oligonucleotide impurities by N-Rich twin-column continuous chromatography, J. Chromatogr. B
N-Rich applied for simultaneous isolation of multiple low-concentration dsRNA impurities enables efficient characterization of oligonucleotide process-related substances.
Peer-Reviewed Publications
16 Feb 2022
Enrichment and purification of peptide impurities using twin-column continuous chromatography, J. Chromatogr. A
N-Rich demonstrated for peptide impurity isolation with up to 15-fold purity improvements and 20-fold mass enrichment compared to conventional methods.
Peer-Reviewed Publications
18 Mar 2021
Using continuous chromatography methodology to achieve high-productivity and high-purity enrichment of charge variants for analytical characterization, J. Chromatogr. A
N-Rich continuous chromatography for mAb charge variant enrichment achieves 95% purity in 10 hours versus 78-87% purity in 48-300 hours with conventional methods.
Peer-Reviewed Publications
28 Jan 2021
A novel twin-column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants, Eng. Life Sci.
N-Rich applied for mAb acidic variant enrichment achieves 86% yield at nearly 100% purity compared to 59% yield with scaled-up batch chromatography.
Educational Articles
Oligo Purification
07 Sep 2026
Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides
Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.
Oligo Purification
27 Aug 2026
Continuous Anion Exchange Purification of a Conjugated siRNA
An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.
Peptide Purification
26 Aug 2026
MCSGP Process Validation: Characterization and PPQ at Commercial Scale
Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.
Peptide Purification
25 Aug 2026
Acetonitrile- and TFA-Free Peptide Purification with MCSGP
Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.
YMC ChromaCon Chromatography Newsletter.
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