Continuous Chromatography Resource Library

Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Brochures & Datasheets 04 Dec 2025 English

Contichrom CUBE Brochure

Batch Chromatography Continuous Capture (CaptureSMB®) Continuous Enrichment (N-Rich®) Continuous Polishing (MCSGP) Dynamic Process Control (AutoPeak® & AutomAb®) Multi-Dimensional Chromatography (2D/3D) System Rentals Demos & Introductions Contichrom® CUBE Antibody-drug Conjugates Impurity Isolation mAbs & Antibody Variants Small Molecules & Nutraceuticals Oligonucleotides Recombinant Proteins Peptides Viral Vectors (AAV)

Peer-Reviewed Publications 15 Dec 2022 English

Obtaining acidic and basic charge variants using a twin-column continuous chromatography system, Protein Expr. Purif.

A twin-column continuous chromatography method enables simultaneous separation and collection of both acidic and basic mAb charge variants in a single continuous operation.

Continuous Enrichment (N-Rich®) Contichrom® CUBE mAbs & Antibody Variants Impurity Isolation Antibody-drug Conjugates

Peer-Reviewed Publications 15 Oct 2022 English

Enrichment and recovery of oligonucleotide impurities by N-Rich twin-column continuous chromatography, J. Chromatogr. B

N-Rich applied for simultaneous isolation of multiple low-concentration dsRNA impurities enables efficient characterization of oligonucleotide process-related substances.

Continuous Enrichment (N-Rich®) Contichrom® CUBE Oligonucleotides Impurity Isolation

Peer-Reviewed Publications 16 Feb 2022 English

Enrichment and purification of peptide impurities using twin-column continuous chromatography, J. Chromatogr. A

N-Rich demonstrated for peptide impurity isolation with up to 15-fold purity improvements and 20-fold mass enrichment compared to conventional methods.

Continuous Enrichment (N-Rich®) Contichrom® CUBE Peptides Impurity Isolation

Peer-Reviewed Publications 18 Mar 2021 English

Using continuous chromatography methodology to achieve high-productivity and high-purity enrichment of charge variants for analytical characterization, J. Chromatogr. A

N-Rich continuous chromatography for mAb charge variant enrichment achieves 95% purity in 10 hours versus 78-87% purity in 48-300 hours with conventional methods.

Continuous Enrichment (N-Rich®) Contichrom® CUBE mAbs & Antibody Variants Impurity Isolation

Peer-Reviewed Publications 28 Jan 2021 English

A novel twin-column continuous chromatography approach for separation and enrichment of monoclonal antibody charge variants, Eng. Life Sci.

N-Rich applied for mAb acidic variant enrichment achieves 86% yield at nearly 100% purity compared to 59% yield with scaled-up batch chromatography.

Continuous Enrichment (N-Rich®) Contichrom® CUBE mAbs & Antibody Variants Impurity Isolation

Educational Articles

Fundamentals of Preparative Chromatography 25 Nov 2025

Part I – What Is Chromatography? The Universal Primer for New Scientists

A practical primer on chromatography: what it is, how it works, and why it matters. Learn about key separation mechanisms including reversed-phase, ion exchange, size exclusion and affinity chromatography, plus how to read chromatograms and the difference between analytical and preparative methods.

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