{"id":1456,"date":"2024-02-01T12:26:00","date_gmt":"2024-02-01T12:26:00","guid":{"rendered":"https:\/\/www.chromacon.com\/?p=1456"},"modified":"2026-05-12T17:35:58","modified_gmt":"2026-05-12T15:35:58","slug":"the-era-of-large-scale-peptide-oligo-purification-with-mcsgp-has-begun","status":"publish","type":"post","link":"https:\/\/www.chromacon.com\/en\/news\/the-era-of-large-scale-peptide-oligo-purification-with-mcsgp-has-begun\/","title":{"rendered":"“The era of large-scale peptide & oligo purification with MCSGP has begun”"},"content":{"rendered":"\n

Z\u00fcrich \u2014February 1st 2024 \u2014<\/strong> YMC ChromaCon celebrates a major industry milestone: Bachem AG has successfully transitioned to large-scale, GMP-compliant purification of peptides and oligonucleotides using Multicolumn Countercurrent Solvent Gradient Purification (MCSGP). This achievement confirms that MCSGP-based continuous chromatography is now a proven production technology for the global TIDES (therapeutic peptides and oligonucleotides) market.<\/p>\n\n\n\n

Bachem\u2019s new manufacturing capability enables purification of up to two tons of crude peptides<\/strong> and 500 grams of oligonucleotides per line<\/strong> under GMP conditions, demonstrating that continuous multicolumn chromatography can meet demanding commercial-scale requirements for quality, productivity, and sustainability.<\/p>\n\n\n\n

https:\/\/www.bachem.com\/knowledge-center\/the-era-of-large-scale-peptide-oligo-purification-with-mcsgp-has-begun\/<\/a><\/p>\n\n\n\n

A New Era for Large-Scale Peptide & Oligonucleotide Purification<\/h2>\n\n\n\n

Purification has long been a bottleneck in TIDES manufacturing. Traditional single-column batch chromatography is often constrained by high solvent consumption, long cycle times, and limited throughput. By implementing MCSGP at industrial scale, Bachem demonstrates that these limitations can be overcome with continuous, digitally controlled processes that deliver high yield and high purity at significantly improved productivity.<\/p>\n\n\n\n

This milestone validates MCSGP as a scalable solution for complex synthetic biomolecules, including peptides, oligonucleotides, and other emerging modalities where downstream processing is historically challenging and expensive.<\/p>\n\n\n\n

How MCSGP Continuous Chromatography Works<\/h2>\n\n\n\n

MCSGP (Multicolumn Countercurrent Solvent Gradient Purification)<\/strong> is a continuous multicolumn chromatography process. Instead of running one overloaded column in batch mode and discarding valuable side fractions, MCSGP operates with two or more columns in a countercurrent arrangement and automatically recycles impurity-containing fractions.<\/p>\n\n\n\n

In a typical MCSGP configuration:<\/p>\n\n\n\n