{"id":1936,"date":"2025-12-03T13:34:02","date_gmt":"2025-12-03T13:34:02","guid":{"rendered":"https:\/\/www.chromacon.com\/?post_type=application&p=1936"},"modified":"2026-05-10T16:13:03","modified_gmt":"2026-05-10T14:13:03","slug":"adc","status":"publish","type":"application","link":"https:\/\/www.chromacon.com\/en\/applications\/adc\/","title":{"rendered":"Antibody-drug Conjugates"},"content":{"rendered":"
\n
\n
\n
\n
\n
\n

\n ADC DOWNSTREAM <\/p>\n \n

\n Narrow DAR. Higher Yield. ADC<\/strong> Purification with MCSGP.\n <\/h1>\n \n
\n

Less cytotoxin wasted. Isolate target DAR species without discarding on-target product to side-fractions \u2014 demonstrated on a Kadcyla\u00ae-type lysine-conjugated ADC model: 100%<\/strong> yield at 57% purity vs. 22%<\/strong> yield at 31% purity for batch (DAR 3.0) | up to 50%<\/strong> fewer synthesis operations (DAR 2.0)<\/p>\n

 <\/p>\n

Source: YMC ChromaCon ADC Application Note (CEX, Kadcyla\u00ae-type model)<\/em><\/strong><\/p>\n <\/div>\n <\/div>\n\n

\n
\n Talk to Our Purification Specialists<\/span><\/a><\/span> <\/div>\n
\n Download the ADC Application Note<\/span><\/a><\/span> <\/div>\n <\/div>\n <\/div>\n <\/div>\n\n
\n
\n
\n \"\" <\/div>\n\n \n
\n
\n Talk to Our Purification Specialists<\/span><\/a><\/span> <\/div>\n
\n Download the ADC Application Note<\/span><\/a><\/span> <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/section>\n\n
\n \n
\n
\n
\n

\n The ADC Purification Challenge: DAR Heterogeneity <\/h2>\n \n
\n

ADC demand is surging \u2014 with over a dozen FDA-approved products, hundreds of clinical candidates, and rapid market growth. But conjugation of cytotoxic payloads to antibody lysine residues or reduced cysteines produces a statistical mixture of DAR species. A narrow DAR distribution is a critical quality attribute<\/strong> \u2014 higher DAR species clear faster and cause more toxicity, while unconjugated antibody (DAR 0) is therapeutically inactive.<\/p>\n

Hydrophobic interaction chromatography (HIC) is the primary preparative tool for DAR control, but the different DAR species have very similar adsorptive properties. Batch HIC forces a tight center-cut, discarding valuable on-target product in the side-fractions. For lysine-conjugated ADCs \u2014 where positional isomers further complicate resolution \u2014 batch yields for a specific DAR can be as low as 22\u201334%.<\/p>\n <\/div>\n \n <\/div>\n\n

\n \"\" \n <\/div>\n <\/div>\n <\/section>\n\n<\/section>\n\n
\n \n
\n
\n
\n

\n The Solution: MCSGP with AutoPeak\u00ae for ADC DAR Homogenization <\/h2>\n \n
\n

MCSGP (Multi-column Countercurrent Solvent Gradient Purification) eliminates the purity-yield trade-off in ADC DAR purification. Two identical chromatographic columns continuously recycle impure DAR side-fractions \u2014 recovering on-target DAR product that batch chromatography would discard. The process uses the same resins, buffers, and gradient conditions<\/strong> as your existing batch ADC method \u2014 whether HIC, CEX, or mixed-mode.<\/p>\n

AutoPeak\u00ae dynamic process control monitors the ADC elution profile in real-time via UV, automatically adjusting collection windows to maintain the target DAR distribution \u2014 enabling robust, unattended DAR polishing runs.<\/p>\n

\n

Same resins. Same buffers. Superior DAR control.<\/strong> MCSGP works with all major HIC, CEX, and mixed-mode resins \u2014 your existing batch ADC conditions are the starting point. Continuous side-fraction recycling recovers on-target DAR product from overlapping peaks, delivering up to 4\u00d7 yield vs. batch. AutoPeak\u00ae compensates in real-time for conjugation variability and column aging, enabling unattended DAR polishing runs.<\/p>\n<\/blockquote>\n <\/div>\n \n Learn About MCSGP with AutoPeak\u00ae<\/span><\/a><\/span> <\/div>\n\n

\n \"\" \n

MSCGP<\/p>\n <\/div>\n <\/div>\n <\/section>\n\n<\/div><\/section>\n\n\n

Proven Results: MCSGP vs. Batch for ADC DAR Purification<\/h2>\n\n\n\n
\n
\n
\n \n \n
\n \n

\n Up to 4\u00d7 <\/h2>\n \n

\n Yield Improvement <\/p>\n \n \n <\/div>\n\n<\/div>\n\n <\/div><\/section><\/div>\n\n\n\n

\n
\n \n \n
\n \n

\n +80% <\/h2>\n \n

\n Productivity Gain <\/p>\n \n \n <\/div>\n\n<\/div>\n\n <\/div><\/section><\/div>\n\n\n\n

\n
\n \n \n
\n \n

\n 55% \u2193 <\/h2>\n \n

\n Buffer Reduction
\r\n <\/p>\n \n \n <\/div>\n\n<\/div>\n\n <\/div><\/section><\/div>\n<\/div>\n\n\n

\n \n
\n \n