{"id":1935,"date":"2025-12-03T13:30:54","date_gmt":"2025-12-03T13:30:54","guid":{"rendered":"https:\/\/www.chromacon.com\/?post_type=application&p=1935"},"modified":"2026-05-10T16:19:00","modified_gmt":"2026-05-10T14:19:00","slug":"recombinant-proteins","status":"publish","type":"application","link":"https:\/\/www.chromacon.com\/en\/applications\/recombinant-proteins\/","title":{"rendered":"Recombinant Proteins"},"content":{"rendered":"
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\n RECOMBINANT PROTEINS <\/p>\n \n

\n Two Steps. One Train. Continuous Recombinant Protein<\/strong> Purification.\n <\/h1>\n \n
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CaptureSMB\u00ae capture and MCSGP polishing on the Contichrom\u00ae platform \u2014 MCSGP demonstrated in collaboration with Bristol Myers Squibb on a PEGylated protein at 98% target purity: +8.2 pp<\/strong> Yield (73.1\u219279.1%) | +17.8%<\/strong> Productivity | \u221215%<\/strong> PMI | lowest COG<\/strong> of all five scenarios modelled. With dual-slope MCSGP: yield further increases to +20\u201323 pp<\/strong> vs. batch maximum. CaptureSMB\u00ae separately validated at 100\u00d7 scale-up<\/strong> by BMS for Protein A capture.<\/p>\n

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Sources: Kim et al., J. Chromatogr. A (2022) \u2014 BMS industrial-scale COG study; Kim et al., J. Chromatogr. A(2023) \u2014 dual-slope MCSGP; CaptureSMB\u00ae, production-scale BMS validation: Angelo et al., BioProcess International 2018<\/em><\/strong><\/p>\n <\/div>\n <\/div>\n\n

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\n Talk to Our Protein Specialists<\/span><\/a><\/span> <\/div>\n
\n Download the BMS Paper<\/span><\/a><\/span> <\/div>\n <\/div>\n <\/div>\n <\/div>\n\n
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\n Talk to Our Protein Specialists<\/span><\/a><\/span> <\/div>\n
\n Download the BMS Paper<\/span><\/a><\/span> <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/div>\n <\/section>\n\n
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\n The Affinity Capture Bottleneck <\/h2>\n \n
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Batch bind-and-elute chromatography leaves resin capacity systematically underutilized. A column is loaded to 60-80% of dynamic binding capacity to avoid product breakthrough \u2014 meaning 20-40% of expensive affinity resin sits idle in every cycle.<\/p>\n

For high-cost resins \u2014 Protein L for F(ab’)\u2082 fragments, Protein A for Fc-fusion proteins, or custom affinity supports for novel scaffolds \u2014 this underutilization directly inflates cost of goods. Add the large buffer volumes required for batch cycling and the long processing times for high-titer feeds, and affinity capture becomes a significant manufacturing bottleneck.<\/p>\n

For manufacturers targeting continuous downstream processing or integration with perfusion bioreactors, batch capture also introduces the largest process time discontinuity in the train.<\/p>\n <\/div>\n \n <\/div>\n\n

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\n The Solution: CaptureSMB\u00ae Continuous Affinity Capture <\/h2>\n \n
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CaptureSMB\u00ae uses a twin-column countercurrent loading strategy to maximize affinity resin utilization. While the first column reaches full saturation, breakthrough is captured on the second column \u2014 ensuring near-complete loading of both columns before any elution occurs. Resin utilization consistently reaches \u226595% versus 60-80% for batch loading.<\/p>\n

AutomAb\u00ae dynamic process control monitors UV breakthrough in real-time and triggers column switching automatically, maintaining optimal loading regardless of feed titer variation \u2014 enabling robust operation directly coupled to a perfusion bioreactor or continuous clarification train.<\/p>\n

CaptureSMB\u00ae is compatible with any affinity, IEX, or mixed-mode capture resin<\/strong> \u2014 Protein A, Protein L, Protein G, and custom affinity ligands.<\/p>\n

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Works with any affinity resin.<\/strong> CaptureSMB\u00ae has been demonstrated for Protein A (mAb and Fc-fusion capture), Protein L \/ KappaSelect (F(ab’)\u2082 and \u03ba-light chain fragment capture), and custom affinity resins. The twin-column principle applies to any bind-and-elute system where maximizing resin utilization and minimizing buffer consumption is valuable.<\/p>\n<\/blockquote>\n <\/div>\n \n Learn About CaptureSMB\u00ae with AutomAb\u00ae<\/span><\/a><\/span> <\/div>\n\n

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Proven Results: CaptureSMB\u00ae vs. Batch – Affinity Capture – F(ab’)\u2082<\/h2>\n\n\n\n
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\n 64% \u2191 <\/h2>\n \n

\n Load
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\n >30% <\/h2>\n \n

\n Resin cost savings (F(ab’)\u2082 \/ Protein L)
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\n 1.9\u00d7 <\/h2>\n \n

\n Productivity
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\n 54% \u2193 <\/h2>\n \n

\n Buffer Consumption
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