RESOURCE LIBRARY

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

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Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Peer-Reviewed Publications 10 Feb 2023

Role of the gradient slope during the product internal recycling for the multicolumn countercurrent solvent gradient purification of PEGylated proteins, J. Chromatogr. A

Investigation of gradient slope effects during MCSGP internal recycling provides insights for process optimization of PEGylated protein separations. J. Chromatogr. A

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 15 Dec 2022

Obtaining acidic and basic charge variants using a twin-column continuous chromatography system, Protein Expr. Purif.

A twin-column continuous chromatography method enables simultaneous separation and collection of both acidic and basic mAb charge variants in a single continuous operation.

Continuous Enrichment (N-Rich®)

Peer-Reviewed Publications 15 Oct 2022

Enrichment and recovery of oligonucleotide impurities by N-Rich twin-column continuous chromatography, J. Chromatogr. B

N-Rich applied for simultaneous isolation of multiple low-concentration dsRNA impurities enables efficient characterization of oligonucleotide process-related substances.

Continuous Enrichment (N-Rich®)

Peer-Reviewed Publications 07 Sep 2022

Design and economic investigation of a Multicolumn Countercurrent Solvent Gradient Purification unit for the separation of an industrially relevant PEGylated protein, J. Chromatogr. A

Economic analysis of MCSGP for industrial PEGylated protein separation establishes cost-benefit frameworks for technology adoption.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 26 Mar 2022

Continuous countercurrent chromatographic twin-column purification of oligonucleotides: The role of the displacement effect, J. Chromatogr. A

Investigation of displacement effects in MCSGP oligonucleotide purification reveals mechanisms that can be exploited to improve process performance through increased loading.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 16 Feb 2022

Enrichment and purification of peptide impurities using twin-column continuous chromatography, J. Chromatogr. A

N-Rich demonstrated for peptide impurity isolation with up to 15-fold purity improvements and 20-fold mass enrichment compared to conventional methods.

Continuous Enrichment (N-Rich®)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

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