Continuous Chromatography Resource Library

Continuous Chromatography Resources — Application Notes, Webinars & Papers

The evidence behind every Contichrom® process. Download the open-access PPQ paper, browse application notes by molecule, watch on-demand webinars, and access the peer-reviewed publications underpinning MCSGP, CaptureSMB®, and N-Rich® at GMP commercial scale.

Recent MCSGP Research Highlights

The first peer-reviewed PC/PPQ framework for MCSGP

The first peer-reviewed PC/PPQ framework for MCSGP. 95% IPC reduction, 73% PMI reduction, 96% gross-to-gross yield — validated at GMP commercial scale on Bachem Bivalirudin.

Eisenhuth, R. & Müller-Späth, T. — Processes, 2025 (Open-Access)

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First peer-reviewed AIEX MCSGP for therapeutic siRNA

First peer-reviewed AIEX MCSGP for therapeutic siRNA. Yield improved from 80% to 93% on a sugar-conjugated siRNA sense strand — software-aided method development enabled fast batch-to-MCSGP transfer. Throughput increased 87%.

Weldon et al. Org. Process Res. Dev. 2025, (Open-Access)

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Proof-of-concept MCSGP for AAV2 polishing

Proof-of-concept MCSGP for AAV2 polishing. Full capsid content increased from 30% to 68% (ddPCR) after six cycles — with +20.6% yield, +23% productivity, and 27% reduced buffer consumption vs batch AEX. AutoPeak® PAT validated against cryo-TEM and ddPCR.

Müller et al.
Biotechnol. Bioeng. (Open-Access)

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Resource Library

Application notes, peer-reviewed papers, process guides, datasheets, and recorded webinars — searchable by application, technology, product, or service.

Peer-Reviewed Publications 16 Dec 2024

Overview and Recent Advances in the Purification and Isolation of Therapeutic Oligonucleotides, Org. Process Res. Dev.

A comprehensive review of traditional and emerging purification technologies for therapeutic oligonucleotides, highlighting MCSGP as a promising approach to overcome yield-purity tradeoffs.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 15 Oct 2024

Integrated multidimensional chromatography on preparative scale for oligonucleotides purification, J. Chromatogr. A

Integrated multidimensional chromatography combining different separation modes enables comprehensive oligonucleotide purification at preparative scale.

Multi-Dimensional Chromatography (2D/3D)

Peer-Reviewed Publications 06 Oct 2024

Model-Supported Design of Multicolumn Countercurrent Solvent Gradient Purification Processes for Rapid Implementation, Ind. Eng. Chem. Res.

A model-supported approach for rapid MCSGP process design reduces experimental burden while enabling quick transfer from batch to continuous chromatography.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 09 Mar 2024

Enhancing the purification of crocin-I from saffron through the combination of multicolumn countercurrent chromatography and green solvents, Anal. Bioanal. Chem.

MCSGP combined with green solvents purifies crocin-I from saffron, demonstrating the technology’s versatility for natural product purification.

Continuous Polishing (MCSGP)

Peer-Reviewed Publications 31 Aug 2023

Continuous Countercurrent Chromatography in Protein Purification, Methods in Molecular Biology, 2023 (Book Chapter)

Comprehensive methodology chapter explaining CaptureSMB and MCSGP concepts, process design, regulatory aspects, scale-up, and UV-based process control with practical application examples.

Continuous Capture (CaptureSMB®)

Peer-Reviewed Publications 01 May 2023

Critical challenges and advances in recombinant adeno-associated virus (rAAV) biomanufacturing, Biotechnol. Bioeng.

A review of current challenges in rAAV manufacturing discussing how continuous chromatography technologies like MCSGP can address full/empty capsid separation.

Continuous Polishing (MCSGP)

Educational Articles

Oligo Purification 07 Sep 2026

Reversed-Phase MCSGP for GalNAc-Conjugated Oligonucleotides

Purification is where oligonucleotide yield is lost, and the synthesis has to be sized to cover it. Converting a reversed-phase batch method for a GalNAc-conjugated gapmer to twin-column MCSGP lifted recovery from 52.7% to 91.6% at the same purity — enough to shrink the synthesis step by 42.5% for the same daily output.

Oligo Purification 27 Aug 2026

Continuous Anion Exchange Purification of a Conjugated siRNA

An established anion exchange batch method for a conjugated siRNA sense strand, converted to continuous twin-column MCSGP: 13 cycles, 27 product elutions, and yield up from 80% to 93% at unchanged purity — plus a software-aided route from batch chromatogram to running method.

Peptide Purification 26 Aug 2026

MCSGP Process Validation: Characterization and PPQ at Commercial Scale

Continuous chromatography is already in production use, but there was no published account of how to validate it for a regulatory filing. This study provides one, working through Process Characterization and Performance Qualification for MCSGP on commercial-scale Bivalirudin purification: 34 parameters assessed, four tested, one critical.

Peptide Purification 25 Aug 2026

Acetonitrile- and TFA-Free Peptide Purification with MCSGP

Green solvents have always cost the peptide industry recovery. This study shows the loss is a property of batch chromatography, not of the solvent: run the same green eluent counter-currently and the yield comes back.

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